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1. Application
4-Nitrophenyl Phosphate (pNPP) is widely used as:
When hydrolyzed by alkaline phosphatase, pNPP produces 4-nitrophenol, yielding a yellow color measurable at ~405 nm.
2. Compliance Standard
(Depending on grade supplied)
3. Product Description
4-Nitrophenyl Phosphate is a chromogenic phosphatase substrate, typically supplied as a white to off-white crystalline powder. It is water-soluble (in salt form) and stable when stored under recommended conditions.
Common chemical forms:
4. Technical Specifications
|
Parameter |
Specification |
|
Chemical Name |
4-Nitrophenyl Phosphate |
|
Synonym |
p-Nitrophenyl Phosphate (pNPP) |
|
Molecular Formula |
C6H4NO6P (free acid) |
|
Molecular Weight |
~219.09 g/mol (free acid) |
|
Appearance |
White to pale yellow crystalline powder |
|
Assay (Purity) |
≥ 98% (AR Grade typical) |
|
Solubility |
Soluble in water (salt form) |
|
Detection Wavelength |
405 nm (after hydrolysis) |
|
Storage |
2–8°C, protected from light |
|
Stability |
Hygroscopic; store tightly closed |
5. Apparatus is Supplied
6. Optional Accessories
7. Common Capacities Available
(Custom bulk packing available for industrial/diagnostic manufacturers.)
8. Dimensions & Weight (Approx.)
(Packaging dependent)
|
Pack Size |
Approx. Bottle Height |
Approx. Weight (Gross) |
|
1 g |
50–60 mm |
80–120 g |
|
5 g |
60–80 mm |
120–180 g |
|
25 g |
80–100 mm |
200–350 g |
|
100 g |
100–120 mm |
400–700 g |
9. Safety & Handling
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