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4 Nitrophenyl Phosphate in MADHYA PRADESH

Leading Manufacturers, Suppliers & Exporters From India


1. Application

4-Nitrophenyl Phosphate (pNPP) is widely used as:

  • Substrate for Alkaline Phosphatase (ALP) assays
  • Enzyme kinetics studies
  • ELISA detection systems (colorimetric substrate)
  • Clinical chemistry reagent for ALP estimation
  • Molecular biology and biochemical research
  • Pharmaceutical quality control laboratories

When hydrolyzed by alkaline phosphatase, pNPP produces 4-nitrophenol, yielding a yellow color measurable at ~405 nm.

2. Compliance Standard

(Depending on grade supplied)

  • AR / ACS Grade
  • ISO 9001 certified manufacturing
  • ISO 13485 (for diagnostic-grade reagents, where applicable)
  • USP / EP / BP grade (if specified)
  • Suitable for IVD use (for diagnostic grade material)

3. Product Description

4-Nitrophenyl Phosphate is a chromogenic phosphatase substrate, typically supplied as a white to off-white crystalline powder. It is water-soluble (in salt form) and stable when stored under recommended conditions.

Common chemical forms:

  • 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate
  • Free acid form (less common)

4. Technical Specifications

Parameter

Specification

Chemical Name

4-Nitrophenyl Phosphate

Synonym

p-Nitrophenyl Phosphate (pNPP)

Molecular Formula

C6H4NO6P (free acid)

Molecular Weight

~219.09 g/mol (free acid)

Appearance

White to pale yellow crystalline powder

Assay (Purity)

≥ 98% (AR Grade typical)

Solubility

Soluble in water (salt form)

Detection Wavelength

405 nm (after hydrolysis)

Storage

2–8°C, protected from light

Stability

Hygroscopic; store tightly closed

5. Apparatus is Supplied

  • 4-Nitrophenyl Phosphate (powder form)
  • Packed in amber glass bottle or HDPE container
  • Supplied with Certificate of Analysis (CoA)
  • Properly labeled with batch number and expiry date

6. Optional Accessories

  • Alkaline Phosphatase enzyme
  • Assay buffers (DEA buffer, Tris buffer)
  • Microplates (96-well ELISA plates)
  • Spectrophotometer (405 nm capability)
  • Micropipettes & tips
  • Analytical balance
  • Desiccator storage

7. Common Capacities Available

  • 1 g
  • 5 g
  • 10 g
  • 25 g
  • 50 g
  • 100 g

(Custom bulk packing available for industrial/diagnostic manufacturers.)

8. Dimensions & Weight (Approx.)

(Packaging dependent)

Pack Size

Approx. Bottle Height

Approx. Weight (Gross)

1 g

50–60 mm

80–120 g

5 g

60–80 mm

120–180 g

25 g

80–100 mm

200–350 g

100 g

100–120 mm

400–700 g

9. Safety & Handling

  • Avoid inhalation and contact with skin/eyes
  • Use PPE (gloves, lab coat, goggles)
  • Store in cool, dry place
  • Refer to SDS for hazard classification

We are providing 4 Nitrophenyl Phosphate in all states

We cover the following Industrial, Commercial & Residential areas:

Agar Malwa
Alirajpur
Anuppur
Ashoknagar
Balaghat
Barwani
Betul
Bhind
Bhopal
Burhanpur
Chhatarpur
Chhindwara
Damoh
Datia
Dewas
Dhar
Dindori
Guna
Gwalior
Harda
Hoshangabad
Indore
Jabalpur
Jhabua
Katni
Khandwa (East Nimar)
Khargone (West Nimar)
Mandla
Mandsaur
Morena
Narsimhapur
Neemuch
Panna
Raisen
Rajgarh
Ratlam
Rewa
Sagar
Satna
Sehore
Seoni
Shahdol
Shajapur
Sheopur
Shivpuri
Sidhi
Singrauli
Tikamgarh
Ujjain
Umaria
Vidisha
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